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Image Search Results
Journal: Life Science Alliance
Article Title: C9ORF72 -derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neurons
doi: 10.26508/lsa.202101276
Figure Lengend Snippet: APOJ protein levels were measured in three distinct healthy lines of iAstrocytes’ conditioned medium. (A) Regression analysis with the Four-parameter logistic curve-fit (4-PL) method was used to determine the best-fit standard curve based on twofold serial dilutions of APOJ standards and absorbance at 450 nm. (B) iAstrocytes were either left untreated or treated for 24 h with various recombinant DPRs at 1 µM (poly-GA 34 fibrils, poly-GA 34 oligomers, poly-PA 50 oligomers, and poly-GP 24 oligomers), and then APOJ protein levels in their conditioned medium were measured and calculated using the previously generated 4-PL standard curve. Bar graphs of mean ± SEM. One-way ANOVA with Tukey’s multiple-comparisons test (control group is “untreated”). * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
Article Snippet: APOJ protein levels were measured using a
Techniques: Recombinant, Generated, Control
Journal: Scientific Reports
Article Title: Clusterin mediates hydroquinone-induced cytotoxic responses in HL-60 differentiated cells
doi: 10.1038/s41598-024-82140-0
Figure Lengend Snippet: CLU protein concentration in HL-60 cells exposed to different concentrations of HQ. CLU protein levels were quantified using ELISA after treatment with HQ at concentrations of 0, 10, 25, and 50 µmol/L ( n = 3 per group). The error bars represent SD, and the mean is shown as horizontal bars. The control group (0 µmol/L HQ) exhibited the highest CLU concentration (19.05 ± 2.32 ng/mL), while HQ-treated groups showed a progressive decrease: 14.45 ± 1.23 ng/mL at 10 µmol/L, 9.43 ± 0.40 ng/mL at 25 µmol/L, and 9.46 ± 0.67 ng/mL at 50 µmol/L. Significant differences were determined by ANOVA followed by Tukey post hoc test (* p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05), ns : not significant.
Article Snippet: The concentration of sCLU proteins was measured using the
Techniques: Protein Concentration, Enzyme-linked Immunosorbent Assay, Control, Concentration Assay
Journal: Scientific Reports
Article Title: Clusterin mediates hydroquinone-induced cytotoxic responses in HL-60 differentiated cells
doi: 10.1038/s41598-024-82140-0
Figure Lengend Snippet: Concentration of sCLU protein in the cell culture supernatant after exposure to varying HQ concentrations. The concentration of sCLU protein in the supernatant of HL -60 cell cultures was determined using ELISA following treatment with HQ at 0, 10, 25, and 50 µmol/L ( n = 3 per group). The control group (0 µmol/L HQ) exhibited the highest concentration (25.88 ± 4.24 ng/mL), while the 10 µmol/L HQ group showed a slight, non-significant decrease (23.73 ± 2.03 ng/mL, p = 0.696). Significant reductions were observed in the 25 µmol/L (17.45 ± 0.36 ng/mL) and 50 µmol/L (12.96 ± 0.67 ng/mL) groups compared to the control (* p < 0.05, *** p < 0.001, ns p > 0.05), ns : not significant.
Article Snippet: The concentration of sCLU proteins was measured using the
Techniques: Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Control